Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins.
Methods Mol Biol
; 1538: 69-82, 2017.
Article
em En
| MEDLINE
| ID: mdl-27943184
ABSTRACT
The chemical synapse displays specialized intercellular adhesion between pre- and potsynaptic plasma membranes mediated by synaptic cell adhesion proteins. In this asymmetric cell adhesion, pre- and postsynapses have their own unique functions; the presynaptic terminal releases neurotransmitter, which diffuses through the synaptic cleft and is received by receptors accumulated at the postsynapse. Such distinct modes of actions of pre- and postsynapses in synaptic neurotransmission are the rate-limiting factors in signal processing in the brain, and thus protein-protein interactions within the pre- and postsynaptic scaffold are of particular importance for brain function by regulating the pre- and postsynaptic function. In the present paper, we outline a method to screen for binding partners of synaptic scaffold proteins biochemically.
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Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Proteínas de Transporte
/
Proteínas Adaptadoras de Transdução de Sinal
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article