Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR.

Hanron, Amelia E; Billman, Zachary P; Seilie, Annette M; Chang, Ming; Murphy, Sean C

Hanron, Amelia E; Billman, Zachary P; Seilie, Annette M; Chang, Ming; Murphy, Sean C.

Afiliação

Hanron AE; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
Billman ZP; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA; Center for Emerging and Re-emerging Infectious Diseases, University of Washington, Seattle, WA, USA.
Seilie AM; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
Chang M; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
Murphy SC; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA; Center for Emerging and Re-emerging Infectious Diseases, University of Washington, Seattle, WA, USA; Department of Microbiology, University of Washington, Seattle, WA, USA. Electronic address: murphysc@uw.edu.

Diagn Microbiol Infect Dis ; 87(3): 226-228, 2017 Mar.

Article em En | MEDLINE | ID: mdl-27986353

RESUMO

Babesia are increasingly appreciated as a cause of transfusion-transmitted infection. Sensitive methods are needed to screen blood products. We report herein that B. microti 18S rRNA is over 1,000-fold more abundant than its coding genes, making reverse transcription PCR (RT-PCR) much more sensitive than PCR. Babesia 18S rRNA may be useful for screening the blood supply.

Assuntos

Babesiose/diagnóstico; DNA de Protozoário/genética; DNA Ribossômico/genética; RNA Ribossômico 18S/genética; Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos; Babesia microti/isolamento & purificação; Infecção Hospitalar/sangue; Infecção Hospitalar/parasitologia; Humanos; Testes Sorológicos/métodos; Reação Transfusional

Palavras-chave

18S rRNA; Babesia; Clinical microbiology; Diagnostics; RT-PCR; Transfusion

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Babesiose / DNA Ribossômico / RNA Ribossômico 18S / DNA de Protozoário / Reação em Cadeia da Polimerase Via Transcriptase Reversa Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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