Validation of the Microreader™ 23sp ID system: A new STR 23-plex system for forensic application.
Forensic Sci Int Genet
; 27: 67-73, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-27992828
ABSTRACT
Microreader™ 23sp ID system is a new 23-plex STR genotyping system that amplified 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290 and D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus in one reaction. Microreader™ 23sp ID system was validated according to the guidelines of "Validation Guidelines for DNA Analysis Methods (2012)" described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. Our results suggested that Microreader™ 23sp ID system is a useful tool for identification and parentage testing.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Impressões Digitais de DNA
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Repetições de Microssatélites
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Reação em Cadeia da Polimerase Multiplex
Limite:
Animals
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Humans
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article