A Sensitive and Label-Free Pb(II) Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex/Thioflavin T Conformation.
Sensors (Basel)
; 16(12)2016 Dec 16.
Article
em En
| MEDLINE
| ID: mdl-27999248
ABSTRACT
Pb(II) can cause serious damaging effects to human health, and thus, the study of Pb2+ detection methods to sensitively and selectively monitor Pb(II) pollution has significant importance. In this work, we have developed a label-free fluorescence sensing strategy based on a Pb(II) DNAzyme cleavage and the ThT/G-quadruplex complex. In the presence of Pb(II), a G-rich tail was cut and released from the substrate strand, which then would form a G-quadruplex structure by combination with ThT dye. The fluorescence signal increase was then measured for sensitive Pb(II) quantification with a limit of detection of 0.06 nM. Our sensor also demonstrated high selectivity against six different metal ions, which is very important for the analysis of complex samples.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
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Tiazóis
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Técnicas Biossensoriais
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DNA Catalítico
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Quadruplex G
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Chumbo
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Conformação Molecular
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article