Prenatal transplantation of epidermal neural crest stem cells in malformation of cortical development mouse model.
Microsc Res Tech
; 80(4): 394-405, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28001323
ABSTRACT
Prenatal interventions may offer an immense opportunity in therapeutic protocols of malformations of cortical development (MCD). Epidermal neural crest stem cells (EPI-NCSCs) of the hair follicle bulge exhibit features of both embryonic and adult stem cells; these cells maintain their neurologic differentiation capability because of their neural crest origin. However, it is unknown if prenatal use of EPI-NCSCs could be beneficial in targeting methylazoxymethanol (MAM)-induced MCD, which further addressed in the present work. EPI-NCSCs were prenatally infused to the MAM-exposed mice. Thicknesses of various cerebral cortex areas as well as corpus callosum was measured; there were markedly decrease in MAM group (p < .001 vs. untreated), but a significant increase in EPI-NCSC group (p < .05 vs. MAM), except for corpus callosum. Real-time PCR analysis showed high expressions for absent, small, or homeotic 2-like protein, nestin, doublecortin (DCX), neuronal specific nuclei protein (NeuN), and glial fibrillary acidic protein (GFAP) in MAM group (p < .001 vs. untreated), except for G-protein-coupled C-X-C chemokine receptor type 4 (CXCR4) and CXC motif ligand 12 (CXCL12), whereas there were low expressions in EPI-NCSCs group (p < .01 vs. MAM). Immunohistochemistry of NeuN, GFAP, ionized calcium-binding adapter molecule (Iba1), and oligodendrocyte lineage transcription factor 2 (Olig2) was also revealed the same pattern as real-time PCR (p < .001 MAM vs. untreated, and p < .05 EPI-NCSCs vs. MAM). Our findings suggest prenatal use of EPI-NCSCs as a possible candidate for cell-based therapy of cortical injury through affecting neural markers and their relationship with glial.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Córtex Cerebral
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Folículo Piloso
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Corpo Caloso
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Neurogênese
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Células-Tronco Neurais
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Terapia Baseada em Transplante de Células e Tecidos
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Crista Neural
Tipo de estudo:
Guideline
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Prognostic_studies
Limite:
Animals
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Pregnancy
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article