Association between higher expression of interleukin-8 (IL-8) and haplotype -353A/-251A/+678T of IL-8 gene with preeclampsia: A case-control study.
Medicine (Baltimore)
; 95(52): e5537, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-28033248
ABSTRACT
Preeclampsia (PE) is a common pregnancy-specific disorder associated with significant maternal and fetal morbidity and mortality worldwide.The present study was performed to investigate the role of a CXC chemokine interleukin-8 (IL-8), in the pathogenesis of PE. IL-8 expression levels were assessed in placental and serum samples from 160 pregnant women with PE (Nâ=â68 severe, 92 mild) and 140 healthy donors.Results from enzyme-linked immunosorbent assay showed that the concentration of serum IL-8 in PE patients (180.27â±â5.81âng/L) was significantly higher than that in healthy controls (41.57â±â5.67âng/L). Patients with severe PE had even higher serum IL-8 levels. Similar messenger RNA and protein expression patterns of IL-8 in placental tissues were confirmed by quantitative real-time polymerase chain reaction and immunohistochemical assay (Nâ=â30 each in the mild PE, severe PE, and control groups). In addition, single nucleotide polymorphisms of IL-8 gene were detected with polymerase chain reaction-restricted fragment length polymorphism/SSP. The frequency of IL-8-251A allele was significantly higher than that in controls (58.4% vs 48.9%, Pâ<â0.05). The occurrence frequency of haplotype -353A/-251A/+678T (AAT) in PE subjects was 27.2% as compared to 21.9% in the control participants (Pâ<â0.05).Our study reveals that IL-8 expression is positively associated with the severity of PE. Presence of haplotype AAT in pregnant women appears to be a risk factor for PE.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Placenta
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Pré-Eclâmpsia
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Interleucina-8
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Adult
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Female
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Humans
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Pregnancy
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article