CaMKII requirement for the persistence of in vivo hippocampal mossy fiber synaptic plasticity and structural reorganization.
Neurobiol Learn Mem
; 139: 56-62, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-28039086
ABSTRACT
CaMKII has been proposed as a molecular substrate for long-term memory storage due to its capacity to maintain an active autophosporylated state even after the decay of the external stimuli. The hippocampal mossy fiber-CA3 pathway (MF-CA3) is considered as a relevant area for acquisition and storage of different learning tasks. MF-CA3 pathway exhibits a form of LTP characterized by a slow initial increase in the EPSP slope that is independent of NMDA receptors activation. Our previous studies show that application of high frequency stimulation sufficient to elicit MF-CA3 LTP produces structural reorganization, in a manner independent of LTP induction, at the stratum oriens of hippocampal CA3 area 7days after stimulation. However, the molecular mechanisms that underlie the maintenance of MF-CA3 LTP as well as the concomitant structural reorganization in this area remain to be elucidated. Here we show that acute microinfusion of myr-CaMKIINtide, a noncompetitive inhibitor of CaMKII, in the hippocampal CA3 area of adult rats during the late-phase of in vivo MF-CA3 LTP blocked its maintenance and prevented the accompanying morphological reorganization in CA3 area. These findings support the idea that CaMKII is a key molecular substrate for the long-term hippocampal synaptic plasticity maintenance.
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MEDLINE
Assunto principal:
Fibras Musgosas Hipocampais
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Inibidores Enzimáticos
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Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina
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Região CA3 Hipocampal
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Plasticidade Neuronal
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article