Metabolomic Profiling in Individuals with a Failing Kidney Allograft.
PLoS One
; 12(1): e0169077, 2017.
Article
em En
| MEDLINE
| ID: mdl-28052095
ABSTRACT
BACKGROUND:
Alteration of certain metabolites may play a role in the pathophysiology of renal allograft disease.METHODS:
To explore metabolomic abnormalities in individuals with a failing kidney allograft, we analyzed by liquid chromatography-mass spectrometry (LC-MS/MS; for ex vivo profiling of serum and urine) and two dimensional correlated spectroscopy (2D COSY; for in vivo study of the kidney graft) 40 subjects with varying degrees of chronic allograft dysfunction stratified by tertiles of glomerular filtration rate (GFR; T1, T2, T3). Ten healthy non-allograft individuals were chosen as controls.RESULTS:
LC-MS/MS analysis revealed a dose-response association between GFR and serum concentration of tryptophan, glutamine, dimethylarginine isomers (asymmetric [A]DMA and symmetric [S]DMA) and short-chain acylcarnitines (C4 and C12), (test for trend T1-T3 = p<0.05; p = 0.01; p<0.001; p = 0.01; p = 0.01; p<0.05, respectively). The same association was found between GFR and urinary levels of histidine, DOPA, dopamine, carnosine, SDMA and ADMA (test for trend T1-T3 = p<0.05; p<0.01; p = 0.001; p<0.05; p = 0.001; p<0.001; p<0.01, respectively). In vivo 2D COSY of the kidney allograft revealed significant reduction in the parenchymal content of choline, creatine, taurine and threonine (all p<0.05) in individuals with lower GFR levels.CONCLUSIONS:
We report an association between renal function and altered metabolomic profile in renal transplant individuals with different degrees of kidney graft function.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Transplante de Rim
/
Metabolômica
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article