Evaluation of cancer cell deformability by microcavity array.
Anal Biochem
; 520: 16-21, 2017 03 01.
Article
em En
| MEDLINE
| ID: mdl-28057503
ABSTRACT
A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 µm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.
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1
Base de dados:
MEDLINE
Assunto principal:
Forma Celular
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article