Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.
Sci Rep
; 7: 39846, 2017 01 06.
Article
em En
| MEDLINE
| ID: mdl-28057919
ABSTRACT
Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Citometria de Fluxo
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Vesículas Extracelulares
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article