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Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.
Atkin-Smith, Georgia K; Paone, Stephanie; Zanker, Damien J; Duan, Mubing; Phan, Than K; Chen, Weisan; Hulett, Mark D; Poon, Ivan K H.
Afiliação
  • Atkin-Smith GK; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
  • Paone S; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
  • Zanker DJ; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
  • Duan M; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
  • Phan TK; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
  • Chen W; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
  • Hulett MD; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
  • Poon IK; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
Sci Rep ; 7: 39846, 2017 01 06.
Article em En | MEDLINE | ID: mdl-28057919
ABSTRACT
Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Apoptose / Citometria de Fluxo / Vesículas Extracelulares Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Apoptose / Citometria de Fluxo / Vesículas Extracelulares Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2017 Tipo de documento: Article