In Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways.
Mol Cell
; 65(1): 39-51, 2017 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-28061332
ABSTRACT
Understanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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RNA Bacteriano
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RNA Mensageiro
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Precursores de RNA
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Salmonella enterica
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Endorribonucleases
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Pequeno RNA não Traduzido
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article