Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies.
Nat Methods
; 14(3): 279-282, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-28092690
ABSTRACT
The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Processamento de Proteína Pós-Traducional
/
Integrase de HIV
/
Anticorpos
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article