ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing.

ABSTRACT

SUMMARY: Spike-in controls are synthetic nucleic-acid sequences that are added to a user's sample and constitute internal standards for subsequent steps in the next generation sequencing workflow. AVAILABILITY AND IMPLEMENTATION The software is implemented in C ++/R and is freely available under BSD license. The source code is available from github.com/student-t/Anaquin , binaries and user manual from www.sequin.xyz/software and R package from bioconductor.org/packages/Anaquin. CONTACT anaquin@garvan.org.au or t.mercer@garvan.org.au. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.