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Evaluating the Protective Effects and Mechanisms of Diallyl Disulfide on Interlukin-1ß-Induced Oxidative Stress and Mitochondrial Apoptotic Signaling Pathways in Cultured Chondrocytes.
Hosseinzadeh, Azam; Jafari, Davood; Kamarul, Tunku; Bagheri, Abolfazll; Sharifi, Ali M.
Afiliação
  • Hosseinzadeh A; RAZI Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
  • Jafari D; Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Kamarul T; Department of Orthopedics Surgery, Bone and Cartilage Reconstruction Joint Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran.
  • Bagheri A; Tissue Engineering Group (NOCERAL), Faculty of Medicine, Department of Orthopedic Surgery, University of Malaya, Kuala Lumpur, Malaysia.
  • Sharifi AM; Department of Orthopedics Surgery, Bone and Cartilage Reconstruction Joint Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran.
J Cell Biochem ; 118(7): 1879-1888, 2017 07.
Article em En | MEDLINE | ID: mdl-28169456
ABSTRACT
The protective effects and mechanisms of DADS on IL-1ß-mediated oxidative stress and mitochondrial apoptosis were investigated in C28I2 human chondrocytes. The effect of various concentrations of DADS (1, 5 10, 25, 50, and 100 µM) on C28I2 cell viability was evaluated in different times (2, 4, 8, 16, and 24 h) to obtain the non-cytotoxic concentrations of drug by MTT-assay. The protective effect of non-toxic concentrations of DADS on experimentally induced oxidative stress and apoptosis by IL-1ß in C28I2 was evaluated. The effects of DADS on IL-1ß-induced intracellular ROS production and lipid peroxidation were detected and the proteins expression of Nrf2, Bax, Bcl-2, caspase-3, total and phosphorylated JNK, and P38 MAPKs were analyzed by Western blotting. The mRNA expression of detoxifying phase II/antioxidant enzymes including heme oxygenase-1, NAD(P)H quinine oxidoreductase, glutathione S-transferase-P1, catalase, superoxide dismutase-1, glutathione peroxidase-1, -3, -4 were evaluated by reverse transcription-polymerase chain reaction. DADS in 1, 5, 10, and 25 µM concentrations had no cytotoxic effect after 24 h. Pretreatment with DADS remarkably increased Nrf2 nuclear translocation as well as the genes expression of detoxifying phase II/antioxidant enzymes and reduced IL-1ß-induced elevation of ROS, lipid peroxidation, Bax/Bcl-2 ratio, caspase-3 activation, and JNK and P38 phosphorylation. DADS could considerably reduce IL-1ß-induced oxidative stress and consequent mitochondrial apoptosis, as the major mechanisms of chondrocyte cell death in an experimental model of osteoarthritis. It may be considered as natural product in protecting OA-induced cartilage damage in clinical setting. J. Cell. Biochem. 118 1879-1888, 2017. © 2017 Wiley Periodicals, Inc.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Estresse Oxidativo / Condrócitos / Dissulfetos / Compostos Alílicos / Interleucina-1beta Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Estresse Oxidativo / Condrócitos / Dissulfetos / Compostos Alílicos / Interleucina-1beta Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article