Effectiveness of repeated photodynamic therapy in the elimination of intracanal Enterococcus faecalis biofilm: an in vitro study.
Lasers Med Sci
; 32(3): 655-661, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28185089
ABSTRACT
The study aimed to investigate the effectiveness of photodynamic therapy in the elimination of intracanal Enterococcus faecalis biofilm and to analyse how a repeated light irradiation, replenishment of oxygen and photosensitiser affect the results of the photodynamic disinfecting protocol. After chemomechanical preparation, 46 single-rooted human teeth were infected with a clinical strain of E. faecalis and incubated for a week in microaerobic conditions. The experimental procedures included groups of single application of photodynamic therapy, two cycles of PDT, irrigation with 5.25% NaOCl solution and negative and positive control. The number of residing bacterial colonies in the root canals was determined based on the CFU/ml method. In the group of preparations irrigated with NaOCl, bacterial colonies were not observed. A single PDT eliminated 45% of the initial CFU/ml. Repeated PDT eradicated 95% of the intracanal bacterial biofilm. Photodynamic therapy has a high potential for the elimination of E. faecalis biofilm. There is a safe therapeutic window where photoinduced disinfection can be used as an adjuvant to conventional endodontic treatment, which remains the most effective.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fotoquimioterapia
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Enterococcus faecalis
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Biofilmes
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Cavidade Pulpar
Tipo de estudo:
Guideline
Limite:
Adult
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Humans
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Male
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article