A semiautomated microfluidic platform for real-time investigation of nanoparticles' cellular uptake and cancer cells' tracking.
Nanomedicine (Lond)
; 12(6): 581-596, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-28186438
ABSTRACT
AIM:
Develop a platform composed of labeled dendrimer nanoparticles (NPs) and a microfluidic device for real-time monitoring of cancer cells fate. MATERIALS &METHODS:
Carboxymethylchitosan/poly(amidoamine) dendrimer NPs were labeled with fluorescein-5(6)-isothiocyanate and characterized using different physicochemical techniques. After, HeLa, HCT-116 and U87MG were cultured in the presence of NPs, and cell viability and internalization efficiency in static (standard culture) and dynamic (microfluidic culture) conditions were investigated.RESULTS:
Cancer cells cultured with NPs in dynamic conditions were viable and presented higher internalization levels as compared with static 2D cultures.CONCLUSION:
This work demonstrated that the proposed microfluidic-based platform allows real-time monitoring, which upon more studies, namely, the assessment of an anticancer drug release effect could be used for cancer theranostics.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Portadores de Fármacos
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Microfluídica
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Nanopartículas
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Neoplasias
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article