Multivalent foldamer-based affinity assay for selective recognition of Aß oligomers.
Anal Chim Acta
; 960: 131-137, 2017 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-28193356
ABSTRACT
Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent ß-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to ß-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10-500 pM. These results suggest that protein mimetic foldamers can be useful tools in biosensors and affinity assays.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
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Peptídeos beta-Amiloides
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Multimerização Proteica
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article