Co-chaperone Hsp70/Hsp90-organizing protein (Hop) is required for transposon silencing and Piwi-interacting RNA (piRNA) biogenesis.
J Biol Chem
; 292(15): 6039-6046, 2017 04 14.
Article
em En
| MEDLINE
| ID: mdl-28193840
ABSTRACT
Piwi-interacting RNAs (piRNAs) are 26-30-nucleotide germ line-specific small non-coding RNAs that have evolutionarily conserved function in mobile genetic element (transposons) silencing and maintenance of genome integrity. Drosophila Hsp70/90-organizing protein homolog (Hop), a co-chaperone, interacts with piRNA-binding protein Piwi and mediates silencing of phenotypic variations. However, it is not known whether Hop has a direct role in piRNA biogenesis and transposon silencing. Here, we show that knockdown of Hop in the germ line nurse cells (GLKD) of Drosophila ovaries leads to activation of transposons. Hop GLKD females can lay eggs at the same rate as wild-type counterparts, but the eggs do not hatch into larvae. Hop GLKD leads to the accumulation of γ-H2Av foci in the germ line, indicating increased DNA damage in the ovary. We also show that Hop GLKD-induced transposon up-regulation is due to inefficient piRNA biogenesis. Based on these results, we conclude that Hop is a critical component of the piRNA pathway and that it maintains genome integrity by silencing transposons.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ovário
/
Fatores de Transcrição
/
Elementos de DNA Transponíveis
/
Inativação Gênica
/
Proteínas de Drosophila
/
RNA Interferente Pequeno
/
Janus Quinases
/
Proteínas Argonautas
/
Células Germinativas
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article