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Self-organizing actin patterns shape membrane architecture but not cell mechanics.
Fritzsche, M; Li, D; Colin-York, H; Chang, V T; Moeendarbary, E; Felce, J H; Sezgin, E; Charras, G; Betzig, E; Eggeling, C.
Afiliação
  • Fritzsche M; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK.
  • Li D; National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Colin-York H; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK.
  • Chang VT; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK.
  • Moeendarbary E; Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.
  • Felce JH; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
  • Sezgin E; Department of Mechanical Engineering, University College London, London WC1E 7JE, UK.
  • Charras G; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK.
  • Betzig E; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK.
  • Eggeling C; London Centre for Nanotechnology and Department of Cell &Developmental Biology, University College London, 17-19 Gordon Street, London WC1H 0AH, UK.
Nat Commun ; 8: 14347, 2017 02 13.
Article em En | MEDLINE | ID: mdl-28194011
ABSTRACT
Cell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic techniques, we here show evidence of such self-organization of the actin cortex in living HeLa cells. During cell adhesion, an active multistage process naturally leads to pattern transitions from actin vortices over stars into asters. This process is primarily driven by Arp2/3 complex nucleation, but not by myosin motors, which is in contrast to what has been theoretically predicted and observed in vitro. Concomitant measurements of mechanics and plasma membrane fluidity demonstrate that changes in actin patterning alter membrane architecture but occur functionally independent of macroscopic cortex elasticity. Consequently, tuning the activity of the Arp2/3 complex to alter filament assembly may thus be a mechanism allowing cells to adjust their membrane architecture without affecting their macroscopic mechanical properties.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Citoesqueleto de Actina / Membrana Celular / Actinas / Fluidez de Membrana Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Citoesqueleto de Actina / Membrana Celular / Actinas / Fluidez de Membrana Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article