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Paper-based microfluidic system for tear electrolyte analysis.
Yetisen, Ali K; Jiang, Nan; Tamayol, Ali; Ruiz-Esparza, Guillermo U; Zhang, Yu Shrike; Medina-Pando, Sofía; Gupta, Aditi; Wolffsohn, James S; Butt, Haider; Khademhosseini, Ali; Yun, Seok-Hyun.
Afiliação
  • Yetisen AK; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com and Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Ma
  • Jiang N; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com and Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Ma
  • Tamayol A; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com and Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Ma
  • Ruiz-Esparza GU; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com and Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Ma
  • Zhang YS; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com and Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Ma
  • Medina-Pando S; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com.
  • Gupta A; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. syun@mgh.harvard.edu.
  • Wolffsohn JS; Ophthalmic Research Group, School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK.
  • Butt H; Nanotechnology Laboratory, School of Engineering, University of Birmingham, Birmingham B15 2TT, UK.
  • Khademhosseini A; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com and Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Ma
  • Yun SH; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. syun@mgh.harvard.edu and Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 65 Landsdowne Street, Cambridge, Massachusetts 021
Lab Chip ; 17(6): 1137-1148, 2017 03 14.
Article em En | MEDLINE | ID: mdl-28207920
The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry eye subclassifications. Here, we demonstrate a portable microfluidic system that allows quantitative analysis of electrolytes in the tear fluid that is suited for point-of-care settings. The microfluidic system consists of a capillary tube for sample collection, a reservoir for sample dilution, and a paper-based microfluidic device for electrolyte analysis. The sensing regions are functionalized with fluorescent crown ethers, o-acetanisidide, and seminaphtorhodafluor that are sensitive to mono- and divalent electrolytes, and their fluorescence outputs are measured with a smartphone readout device. The measured sensitivity values of Na+, K+, Ca2+ ions and pH in artificial tear fluid were matched with the known ion concentrations within the physiological range. The microfluidic system was tested with samples having different ionic concentrations, demonstrating the feasibility for the detection of early-stage dry eye, differential diagnosis of dry eye sub-types, and their severity staging.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Lágrimas / Técnicas Analíticas Microfluídicas / Eletrólitos Tipo de estudo: Screening_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Lágrimas / Técnicas Analíticas Microfluídicas / Eletrólitos Tipo de estudo: Screening_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article