Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration.
J Clin Periodontol
; 44(5): 530-539, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-28207939
ABSTRACT
AIM:
The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. MATERIALS ANDMETHODS:
Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray.RESULTS:
Chemokines were categorized into three groups by specific patterns of "appearing," "increasing," and "decreasing/disappearing" from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion.CONCLUSION:
The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Ligamento Periodontal
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Periodontite
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Células-Tronco
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Periodonto
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Movimento Celular
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Quimiocinas
Limite:
Adult
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article