Distinct conformations of GPCR-ß-arrestin complexes mediate desensitization, signaling, and endocytosis.
Proc Natl Acad Sci U S A
; 114(10): 2562-2567, 2017 03 07.
Article
em En
| MEDLINE
| ID: mdl-28223524
ABSTRACT
ß-Arrestins (ßarrs) interact with G protein-coupled receptors (GPCRs) to desensitize G protein signaling, to initiate signaling on their own, and to mediate receptor endocytosis. Prior structural studies have revealed two unique conformations of GPCR-ßarr complexes the "tail" conformation, with ßarr primarily coupled to the phosphorylated GPCR C-terminal tail, and the "core" conformation, where, in addition to the phosphorylated C-terminal tail, ßarr is further engaged with the receptor transmembrane core. However, the relationship of these distinct conformations to the various functions of ßarrs is unknown. Here, we created a mutant form of ßarr lacking the "finger-loop" region, which is unable to form the core conformation but retains the ability to form the tail conformation. We find that the tail conformation preserves the ability to mediate receptor internalization and ßarr signaling but not desensitization of G protein signaling. Thus, the two GPCR-ßarr conformations can carry out distinct functions.
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MEDLINE
Assunto principal:
Receptores Acoplados a Proteínas G
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Endocitose
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Proteínas Mutantes
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Beta-Arrestinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article