Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote.
Epigenetics Chromatin
; 10: 5, 2017.
Article
em En
| MEDLINE
| ID: mdl-28228845
ABSTRACT
BACKGROUND:
In the mammalian zygote, epigenetic reprogramming is a tightly controlled process of coordinated alterations of histone and DNA modifications. The parental genomes of the zygote show distinct patterns of histone H3 variants and distinct patterns of DNA and histone modifications. The molecular mechanisms linking histone variant-specific modifications and DNA methylation reprogramming during the first cell cycle remain to be clarified.RESULTS:
Here, we show that the degree and distribution of H3K9me2 and of DNA modifications (5mC/5hmC) are influenced by the phosphorylation status of H3S10 and H3T11. The overexpression of the mutated histone variants H3.1 and 3.2 at either serine 10 or threonine 11 causes a decrease in H3K9me2 and 5mC and a concomitant increase in 5hmC in the maternal genome. Bisulphite sequencing results indicate an increase in hemimethylated CpG positions following H3.1T10A overexpression suggesting an impact of H3S10 and H3T11 phosphorylation on DNA methylation maintenance.CONCLUSIONS:
Our data suggest a crosstalk between the cell-cycle-dependent control of S10 and T11 phosphorylation of histone variants H3.1 and H3.2 and the maintenance of the heterochromatic mark H3K9me2. This histone H3 "phospho-methylation switch" also influences the oxidative control of DNA methylation in the mouse zygote.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Serina
/
Treonina
/
Histonas
/
Metilação de DNA
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article