Synthesis of antisense oligonucleotides containing acyclic alkynyl nucleoside analogs and their biophysical and biological properties.
Bioorg Med Chem
; 25(7): 2035-2042, 2017 04 01.
Article
em En
| MEDLINE
| ID: mdl-28242172
ABSTRACT
The synthesis of oligonucleotide (ON) analogs, which can be used as antisense molecules, has recently gained much attention. Here, we report the synthesis and properties of an ON analog containing acyclic thymidine and cytidine analogs with a 4-pentyl-1,2-diol instead of the d-ribofuranose moiety. The incorporation of these analogs into the ON improved its nuclease resistance to 3'-exonucleases. Furthermore, it was found that the incorporation of the acyclic thymidine analog into a DNA/RNA duplex accelerates the RNA cleavage of a DNA/RNA duplex by Escherichia coli RNase H.
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Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos Antissenso
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article