Isolation of Mouse Stromal Vascular Cells for Monolayer Culture.
Methods Mol Biol
; 1566: 9-16, 2017.
Article
em En
| MEDLINE
| ID: mdl-28244036
ABSTRACT
Positive energy balance contributes to adipose tissue expansion and dysfunction, which accounts largely for obesity and related metabolic disorders. Thermogenic fat can dissipate energy, activation or induction of which may promote energy balance and address the pressing health issues. Recent studies have shown that stromal vascular fraction (SVF) from white adipose tissue (WAT) can develop both white and brown-like adipocyte phenotypes, thus serving as a unique model to study adipogenesis and thermogenesis. Here, we describe a protocol for effective isolation of mouse SVF from WAT, induction of differentiation, and detection of adipogenesis. Success tips for isolation and culture of SVF are also discussed.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Células Estromais
/
Técnicas de Cultura de Células
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Tecido Adiposo Branco
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article