Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems.

Ma, Su; Preims, Marita; Piumi, François; Kappel, Lisa; Seiboth, Bernhard; Record, Eric; Kracher, Daniel; Ludwig, Roland

Ma, Su; Preims, Marita; Piumi, François; Kappel, Lisa; Seiboth, Bernhard; Record, Eric; Kracher, Daniel; Ludwig, Roland.

Afiliação

Ma S; Department of Food Sciences and Technology, Vienna Institute of Biotechnology, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.
Preims M; Department of Food Sciences and Technology, Vienna Institute of Biotechnology, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.
Piumi F; UMR BDR, INRA, ENVA, Université Paris Saclay, 78350, Jouy en Josas, France.
Kappel L; Research Area Biochemical Technology, Institute of Chemical Engineering, TU Wien, Gumpendorferstrasse 1a, Vienna, Austria.
Seiboth B; Research Area Biochemical Technology, Institute of Chemical Engineering, TU Wien, Gumpendorferstrasse 1a, Vienna, Austria.
Record E; Aix Marseille Université, INRA, BBF (Biodiversité et Biotechnologie Fongiques), Marseille, France.
Kracher D; Department of Food Sciences and Technology, Vienna Institute of Biotechnology, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.
Ludwig R; Department of Food Sciences and Technology, Vienna Institute of Biotechnology, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria. roland.ludwig@boku.ac.at.

Microb Cell Fact ; 16(1): 37, 2017 Feb 28.

Article em En | MEDLINE | ID: mdl-28245812

Assuntos

Aspergillus niger/genética; Desidrogenases de Carboidrato/genética; Desidrogenases de Carboidrato/metabolismo; Escherichia coli/genética; Expressão Gênica; Trichoderma/genética; Aspergillus niger/enzimologia; Desidrogenases de Carboidrato/isolamento & purificação; Catálise; Meios de Cultura/química; Estabilidade Enzimática; Escherichia coli/enzimologia; Glicosilação; Cinética; Pichia/enzimologia; Pichia/genética; Proteínas Recombinantes/metabolismo; Sordariales/enzimologia; Temperatura; Trichoderma/enzimologia

Palavras-chave

Aspergillus niger; Cellobiose dehydrogenase; Cofactor loading; Escherichia coli; Glycoforms; Heterologous expression; Pichia pastoris; Trichoderma reesei

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aspergillus niger / Trichoderma / Desidrogenases de Carboidrato / Expressão Gênica / Escherichia coli Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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