Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide.
Chemistry
; 23(20): 4765-4769, 2017 Apr 06.
Article
em En
| MEDLINE
| ID: mdl-28256754
ABSTRACT
Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte-specific switch-on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide-nucleic-acid-based linker. The ROS-sensing ability was assessed and validated in cell-free assays and in cell culture.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Superóxidos
/
Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article