Transcription factor Wilms' tumor 1 regulates developmental RNAs through 3' UTR interaction.
Genes Dev
; 31(4): 347-352, 2017 02 15.
Article
em En
| MEDLINE
| ID: mdl-28289143
ABSTRACT
Wilms' tumor 1 (WT1) is essential for the development and homeostasis of multiple mesodermal tissues. Despite evidence for post-transcriptional roles, no endogenous WT1 target RNAs exist. Using RNA immunoprecipitation and UV cross-linking, we show that WT1 binds preferentially to 3' untranslated regions (UTRs) of developmental targets. These target mRNAs are down-regulated upon WT1 depletion in cell culture and developing kidney mesenchyme. Wt1 deletion leads to rapid turnover of specific mRNAs. WT1 regulates reporter gene expression through interaction with 3' UTR-binding sites. Combining experimental and computational analyses, we propose that WT1 influences key developmental and disease processes in part through regulating mRNA turnover.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Tumor de Wilms
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Regulação da Expressão Gênica no Desenvolvimento
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Regiões 3' não Traduzidas
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article