Protease Substrate Profiling by N-Terminal COFRADIC.
Methods Mol Biol
; 1574: 51-76, 2017.
Article
em En
| MEDLINE
| ID: mdl-28315243
ABSTRACT
Detection of (neo-)N-terminal peptides is essential for identifying protease cleavage sites . We here present an update of a well-established and efficient selection method for enriching N-terminal peptides out of peptide mixtures N-terminal COFRADIC (COmbined FRActional DIagonal Chromatography). This method is based on the old concept of diagonal chromatography, which involves a peptide modification step in between otherwise identical chromatographic separations, with this modification step finally allowing for the isolation of N-terminal peptides by longer retention of non-N-terminal peptides on the resin. N-terminal COFRADIC has been successfully applied in many protease-centric studies, as well as for studies on protein alpha-N-acetylation and on characterizing alternative translation initiation events.
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MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Endopeptidases
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Cromatografia Líquida
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Proteoma
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Proteômica
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Espectrometria de Massas em Tandem
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article