Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake.
Int J Parasitol
; 47(6): 327-345, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-28315362
ABSTRACT
To identify trematode diversity and life-cycles in the sub-Arctic Lake Takvatn, Norway, we characterised 120 trematode isolates from mollusc first intermediate hosts, metacercariae from second intermediate host fishes and invertebrates, and adults from fish and invertebrate definitive hosts, using molecular techniques. Phylogenies based on nuclear and/or mtDNA revealed high species richness (24 species or species-level genetic lineages) and uncovered trematode diversity (16 putative new species) from five families typical in lake ecosystems (Allocreadiidae, Diplostomidae, Plagiorchiidae, Schistosomatidae and Strigeidae). Sampling potential invertebrate hosts allowed matching of sequence data for different stages, thus achieving molecular elucidation of trematode life-cycles and exploration of host-parasite interactions. Phylogenetic analyses also helped identify three major mollusc intermediate hosts (Radix balthica, Pisidium casertanum and Sphaerium sp.) in the lake. Our findings increase the known trematode diversity at the sub-Arctic Lake Takvatn, showing that digenean diversity is high in this otherwise depauperate sub-Arctic freshwater ecosystem and indicating that sub-Arctic and Arctic ecosystems may be characterised by unique trematode assemblages.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trematódeos
/
Infecções por Trematódeos
/
Variação Genética
/
Lagos
/
Doenças dos Peixes
/
Invertebrados
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País como assunto:
Europa
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article