Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function.
Am J Gastroenterol
; 112(6): 913-923, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28323272
ABSTRACT
OBJECTIVES:
The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function.METHODS:
A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression.RESULTS:
There were no differences in the in vivo measurements of barrier function between IBS-C patients and healthy controls cumulative excretion of 13C mannitol (0-2 h mean (s.e.m.); IBS-C 12.1 (0.9) mg vs. healthy 13.2 (0.8) mg) and lactulose (8-24 h; IBS-C 0.9 (0.5) mg vs. healthy 0.5 (0.2) mg); duodenal impedance IBS-C 729 (65) Ω vs. healthy 706 (43) Ω; plasma mean endotoxin activity level IBS-C 0.36 (0.03) vs. healthy 0.35 (0.02); and in colonic mRNA expression of occludin, zonula occludens (ZO) 1-3, and claudins 1-12 and 14-19. The ex vivo findings were consistent, with no group differences duodenal TMR (IBS-C 28.2 (1.9) Ω cm2 vs. healthy 29.8 (1.9) Ω cm2) and colonic TMR (IBS-C 19.1 (1.1) Ω cm2 vs. healthy 17.6 (1.7) Ω cm2); fluorescein isothiocyanate (FITC)-dextran (4 kDa) and E. coli Bio-Particle flux. Colonic basal Isc was similar, but duodenal basal Isc was lower in IBS-C (43.5 (4.5) µA cm-2) vs. healthy (56.9 (4.9) µA cm-2), P=0.05. Ach-evoked ΔIsc was similar.CONCLUSIONS:
Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Colo
/
Síndrome do Intestino Irritável
/
Duodeno
/
Mucosa Intestinal
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Lactulose
/
Manitol
Tipo de estudo:
Etiology_studies
/
Observational_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
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Humans
/
Middle aged
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article