Combined spiroergometry and 31 P-MRS of human calf muscle during high-intensity exercise.
NMR Biomed
; 30(7)2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28340292
ABSTRACT
Simultaneous measurements of pulmonary oxygen consumption (VO2 ), carbon dioxide exhalation (VCO2 ) and phosphorus magnetic resonance spectroscopy (31 P-MRS) are valuable in physiological studies to evaluate muscle metabolism during specific loads. Therefore, the aim of this study was to adapt a commercially available spirometric device to enable measurements of VO2 and VCO2 whilst simultaneously performing 31 P-MRS at 3 T. Volunteers performed intense plantar flexion of their right calf muscle inside the MR scanner against a pneumatic MR-compatible pedal ergometer. The use of a non-magnetic pneumotachograph and extension of the sampling line from 3 m to 5 m to place the spirometric device outside the MR scanner room did not affect adversely the measurements of VO2 and VCO2 . Response and delay times increased, on average, by at most 0.05 s and 0.79 s, respectively. Overall, we were able to demonstrate a feasible ventilation response (VO2 = 1.05 ± 0.31 L/min; VCO2 = 1.11 ± 0.33 L/min) during the exercise of a single calf muscle, as well as a good correlation between local energy metabolism and muscular acidification (τPCr fast and pH; R2 = 0.73, p < 0.005) and global respiration (τPCr fast and VO2 ; R2 = 0.55, p = 0.01). This provides improved insights into aerobic and anaerobic energy supply during strong muscular performances.
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Base de dados:
MEDLINE
Assunto principal:
Consumo de Oxigênio
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Fósforo
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Espirometria
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Oximetria
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Espectroscopia de Ressonância Magnética
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Ergometria
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Músculo Esquelético
Limite:
Adult
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Humans
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Male
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article