Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop.
Elife
; 62017 03 27.
Article
em En
| MEDLINE
| ID: mdl-28346135
ABSTRACT
The synaptonemal complex (SC) is an ultrastructurally conserved proteinaceous structure that holds homologous chromosomes together and is required for the stabilization of pairing interactions and the completion of crossover (CO) formation between homologs during meiosis I. Here, we identify a novel role for a central region component of the SC, SYP-4, in negatively regulating formation of recombination-initiating double-strand breaks (DSBs) via a feedback loop triggered by crossover designation in C. elegans. We found that SYP-4 is phosphorylated dependent on Polo-like kinases PLK-1/2. SYP-4 phosphorylation depends on DSB formation and crossover designation, is required for stabilizing the SC in pachytene by switching the central region of the SC from a more dynamic to a less dynamic state, and negatively regulates DSB formation. We propose a model in which Polo-like kinases recognize crossover designation and phosphorylate SYP-4 thereby stabilizing the SC and making chromosomes less permissive for further DSB formation.
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MEDLINE
Assunto principal:
Proteínas Nucleares
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Processamento de Proteína Pós-Traducional
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Proteínas Serina-Treonina Quinases
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Caenorhabditis elegans
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Proteínas de Caenorhabditis elegans
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Retroalimentação Fisiológica
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Quebras de DNA de Cadeia Dupla
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article