Monitoring the Disassembly of Virus-like Particles by 19F-NMR.
J Am Chem Soc
; 139(15): 5277-5280, 2017 04 19.
Article
em En
| MEDLINE
| ID: mdl-28350443
ABSTRACT
Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of 19F-labeled VLPs derived from bacteriophage Qß by 19F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disassembled, as well as detection of a degraded state upon digestion by cells. This in turn allowed mutational redesign of disassembly and testing in both bacterial and mammalian systems as a strategy for the creation of putative, targeted-VLP delivery systems.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
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Ressonância Magnética Nuclear Biomolecular
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Vacinas de Partículas Semelhantes a Vírus
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Flúor
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article