Selective Enzymatic Demethylation of N<sup>2</sup> ,N<sup>2</sup> -Dimethylguanosine in RNA and Its Application in High-Throughput tRNA Sequencing.

Dai, Qing; Zheng, Guanqun; Schwartz, Michael H; Clark, Wesley C; Pan, Tao

Selective Enzymatic Demethylation of N² ,N² -Dimethylguanosine in RNA and Its Application in High-Throughput tRNA Sequencing.

Dai, Qing; Zheng, Guanqun; Schwartz, Michael H; Clark, Wesley C; Pan, Tao.

Afiliação

Dai Q; Department of Chemistry, The University of Chicago, Chicago, IL, 60637, USA.
Zheng G; Department of Biochemistry & Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.
Schwartz MH; Department of Biochemistry & Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.
Clark WC; Department of Biochemistry & Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.
Pan T; Department of Biochemistry & Molecular Biology, The University of Chicago, Chicago, IL, 60637, USA.

Angew Chem Int Ed Engl ; 56(18): 5017-5020, 2017 04 24.

Article em En | MEDLINE | ID: mdl-28371071

Assuntos

Enzimas AlkB/metabolismo; Escherichia coli/enzimologia; Guanosina/análogos & derivados; Sequenciamento de Nucleotídeos em Larga Escala/métodos; RNA de Transferência/análise; Enzimas AlkB/genética; Desmetilação; Escherichia coli/genética; Escherichia coli/metabolismo; Guanosina/metabolismo; Modelos Moleculares; Mutação; RNA/análise; RNA/metabolismo; RNA de Transferência/metabolismo

Palavras-chave

AlkB mutant; demethylase; methylguanosine; reverse transcriptase reaction; tRNA sequencing

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA de Transferência / Escherichia coli / Sequenciamento de Nucleotídeos em Larga Escala / Enzimas AlkB / Guanosina Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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