Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposinâ
D.
Angew Chem Int Ed Engl
; 56(19): 5252-5257, 2017 05 02.
Article
em En
| MEDLINE
| ID: mdl-28378443
ABSTRACT
The main glycoforms of the hydrophobic lysosomal glycoprotein saposinâ
D (SapD) were synthesized by native chemical ligation. An approach for the challenging solid-phase synthesis of the fragments was developed. Three SapD glycoforms were obtained following a general and robust refolding and purification protocol. A crystal structure of one glycoform confirmed its native structure and disulfide pattern. Functional assays revealed that the lipid-binding properties of three SapD glycoforms are highly affected by the single sugar moiety of SapD showing a dependency of the size and the type of N-glycan.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carboidratos
/
Saposinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article