Your browser doesn't support javascript.
loading
Serodiagnosis of dengue virus infection using commercially available antibody and NS1 antigen ELISAs.
Granger, D; Leo, Y S; Lee, L K; Theel, E S.
Afiliação
  • Granger D; Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
  • Leo YS; Institute of Infectious Diseases and Epidemiology, Communicable Disease Centre, Tan Tock Seng Hospital, Singapore.
  • Lee LK; Institute of Infectious Diseases and Epidemiology, Communicable Disease Centre, Tan Tock Seng Hospital, Singapore.
  • Theel ES; Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA. Electronic address: theel.elitza@mayo.edu.
Diagn Microbiol Infect Dis ; 88(2): 120-124, 2017 Jun.
Article em En | MEDLINE | ID: mdl-28389145
Accuracy of the InBios DENV Detect IgM, IgG and NS1 antigen (Ag) ELISAs (Seattle, WA) for detection of dengue virus (DENV) infection were evaluated using 100 retrospectively selected sera from acutely febrile patients presenting to a Singapore hospital. The InBios DENV NS1, IgM and IgG ELISAs had an overall sensitivity of 83.6%, 40% and 58.2% and an overall specificity of 97.8%, 97.8% and 55.6%, respectively. Simultaneous testing for NS1 and IgM-antibodies yielded a sensitivity and specificity of 85.5% and 95.5%, respectively, which did not significantly differ from testing for NS1 Ag alone. Using sera positive for IgM- or IgG-class antibodies to six common arboviruses, the InBios IgM and IgG ELISAs showed an overall analytic specificity of 89.2% and 66.4%, respectively. This study suggests that recent DENV infection can reliably be detected by the InBios NS1 Ag ELISA alone and that InBios DENV IgG reactivity should be interpreted with caution.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Imunoglobulina M / Ensaio de Imunoadsorção Enzimática / Proteínas não Estruturais Virais / Dengue / Anticorpos Antivirais / Antígenos Virais Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País como assunto: Asia Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Imunoglobulina M / Ensaio de Imunoadsorção Enzimática / Proteínas não Estruturais Virais / Dengue / Anticorpos Antivirais / Antígenos Virais Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País como assunto: Asia Idioma: En Ano de publicação: 2017 Tipo de documento: Article