Enhanced immunogenicity following miR-155 incorporation into the influenza A virus genome.
Virus Res
; 235: 115-120, 2017 05 02.
Article
em En
| MEDLINE
| ID: mdl-28392443
ABSTRACT
Influenza A vaccine efficacy in the elderly is generally poor and so identification of novel molecular adjuvants to improve immunogenicity is important to reduce the overall burden of disease. Short non-coding RNAs, known as microRNAs (miRNAs) are known to regulate gene expression and have the potential to influence immune responses. One such miRNA, miR-155, has been shown to modulate T and B cell development and function. We incorporated miR-155 into the influenza A virus (IAV) genome creating a self-adjuvanting 'live vaccine' with the ability to modify immunogenicity. Infection of mice with a recombinant influenza virus encoding miR-155 in the NS gene segment altered epitope-specific expansion of influenza-specific CD8+ T cells and induced significantly higher levels of neutralising antibody.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
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Genoma Viral
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Linfócitos T CD8-Positivos
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MicroRNAs
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Anticorpos Neutralizantes
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Anticorpos Antivirais
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article