Optimization of bioengineered heparin/heparan sulfate production for therapeutic applications.
Bioengineered
; 8(5): 661-664, 2017 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-28394734
ABSTRACT
Heparin has been used clinically as an anti-coagulant for more than 100 y and the major source of this therapeutic is still animal tissues. Contamination issues in some batches of heparin over 10 y ago have highlighted the need to develop alternative methods of production of this essential drug. 1 Bioengineering heparin by expressing serglycin in mammalian cells is a promising approach that was recently reported by the authors. 2 This addendum explores the approaches that the authors are taking to increase the yield of recombinantly expressed serglycin decorated with heparin/heparan sulfate focusing on cell culture and bioreactor conditions and proposes that the cell microenvironment is a key modulator of heparin biosynthesis.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
Heparina
/
Melhoramento Genético
/
Proteínas de Transporte Vesicular
/
Glucose
/
Heparitina Sulfato
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article