A highly efficient method for extracting next-generation sequencing quality RNA from adipose tissue of recalcitrant animal species.
J Cell Physiol
; 233(3): 1971-1974, 2018 Mar.
Article
em En
| MEDLINE
| ID: mdl-28407290
ABSTRACT
The next-generation sequencing (NGS) based RNA sequencing (RNA-Seq) and transcriptome profiling offers an opportunity to unveil complex biological processes. Successful RNA-Seq and transcriptome profiling requires a large amount of high-quality RNA. However, NGS-quality RNA isolation is extremely difficult from recalcitrant adipose tissue (AT) with high lipid content and low cell numbers. Further, the amount and biochemical composition of AT lipid varies depending upon the animal species which can pose different degree of resistance to RNA extraction. Currently available approaches may work effectively in one species but can be almost unproductive in another species. Herein, we report a two step protocol for the extraction of NGS quality RNA from AT across a broad range of animal species.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA
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Extratos Celulares
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Tecido Adiposo
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Extração em Fase Sólida
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article