Characterization of T-Dependent and T-Independent B Cell Responses to a Virus-like Particle.
J Immunol
; 198(10): 3846-3856, 2017 05 15.
Article
em En
| MEDLINE
| ID: mdl-28416599
ABSTRACT
Natural pathogens, such as viruses, often induce T-dependent and T-independent Ab responses. However, the activation and differentiation of Ag-specific B cells under these conditions had not been examined in detail. In this study, we used bacterial phage Qß-derived virus-like particles (Qß-VLPs) as an immunogen to examine the T-independent and T-dependent phases of the response in mice. Using Qß-specific cell labeling and enrichment methods developed in this study, we were able to characterize the rare Ag-specific B cells in detail. Surprisingly, we found that Qß-VLPs could induce Bcl-6 expression in pregerminal center B cells independently of T cell help. In addition, Qß-VLP-induced T-independent responses could lead to isotype-switched and somatically mutated memory B cells. Finally, in contrast to what has been reported with several other Ags, long-lived IgG+ memory cells were induced by Qß-VLPs, with IgM+ memory B cells being produced but only evident for a limited time, suggesting that different types of immunogens may preferentially generate or maintain IgM+ versus IgG+ memory B cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
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Linfócitos T
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Vacinas de Partículas Semelhantes a Vírus
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Memória Imunológica
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article