Crystal structure of the EnvZ periplasmic domain with CHAPS.
FEBS Lett
; 591(10): 1419-1428, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-28423182
Bacteria sense and respond to osmolarity through the EnvZ-OmpR two-component system. The structure of the periplasmic sensor domain of EnvZ (EnvZ-PD) is not available yet. Here, we present the crystal structure of EnvZ-PD in the presence of CHAPS detergent. The structure of EnvZ-PD shows similar folding topology to the PDC domains of PhoQ, DcuS, and CitA, but distinct orientations of helices and ß-hairpin structures. The CD and NMR spectra of EnvZ-PD in the presence of cholate, a major component of bile salts, are similar to those with CHAPS. Chemical cross-linking shows that the dimerization of EnvZ-PD is significantly inhibited by the CHAPS and cholate. Together with ß-galactosidase assay, these results suggest that bile salts may affect the EnvZ structure and function in Escherichia coli.
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MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
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Ácidos Cólicos
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Colatos
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Proteínas de Escherichia coli
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Detergentes
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Escherichia coli
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Complexos Multienzimáticos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article