Landscape of RNA polyadenylation in E. coli.
Nucleic Acids Res
; 45(5): 2746-2756, 2017 03 17.
Article
em En
| MEDLINE
| ID: mdl-28426097
ABSTRACT
Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5Î-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and from a poly(A)polymerase deleted mutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A)polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A)polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3Î-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments.
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Base de dados:
MEDLINE
Assunto principal:
Polinucleotídeo Adenililtransferase
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RNA Bacteriano
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Regulação Bacteriana da Expressão Gênica
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Proteínas de Escherichia coli
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Poliadenilação
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Escherichia coli
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article