Design and validation of a new method to detect and quantify residual host cell DNA in human recombinant erythropoietin (rEPO).

Zamanian, Shima; Mohammadi-Yeganeh, Samira; Kia, Vahid; Kaghazian, Hooman; Paryan, Mahdi

Zamanian, Shima; Mohammadi-Yeganeh, Samira; Kia, Vahid; Kaghazian, Hooman; Paryan, Mahdi.

Afiliação

Zamanian S; a Faculty of Science, University of Guilan , Department of Biology , Rasht , Iran.
Mohammadi-Yeganeh S; b Cellular and Molecular Biology Research Center , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
Kia V; c Department of Biotechnology, School of Advanced Technologies in Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
Kaghazian H; d Faculty of Medicine, Department of Medical Biotechnology , Zanjan University of Medical Sciences , Zanjan , Iran.
Paryan M; e Department of Research and Development, Production and Research Complex , Pasteur Institute of Iran , Tehran , Iran.

Prep Biochem Biotechnol ; 47(9): 847-851, 2017 Oct 21.

Article em En | MEDLINE | ID: mdl-28426392

Assuntos

DNA/análise; Eritropoetina/química; Proteínas Recombinantes/química; Clonagem Molecular/métodos; DNA/genética; Eritropoetina/genética; Escherichia coli/genética; Humanos; Reação em Cadeia da Polimerase em Tempo Real/métodos; Proteínas Recombinantes/genética

Palavras-chave

Biopharmaceuticals; human recombinant EPO; residual DNA

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / DNA / Eritropoetina Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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