UHPLC-Q-TOF-MS/MS-based screening and characterization of metabolites of cnidilin in human liver microsomes.
Biomed Chromatogr
; 31(11)2017 Nov.
Article
em En
| MEDLINE
| ID: mdl-28431455
ABSTRACT
Cnidilin is an active natural furocoumarin ingredient originating from well-known traditional Chinese medicine Radix Angelicae Dahuricae. In the present study, an efficient approach was developed for the screening and identification of cnidilin metabolites using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. In this approach, an on-line data acquisition method multiple mass defect filter combined with dynamic background subtraction was developed to trace all probable metabolites. Based on this analytical strategy, a total of 24 metabolites of cnidilin were detected in human liver microsomal incubation samples and the metabolic pathways were proposed. The results indicated that oxidation was the main biotransformation route for cnidilin in human liver microsomes. In addition, the specific cytochrome P450 (CYP) enzymes involved in the metabolism of cnidilin were identified using chemical inhibition and CYP recombinant enzymes. The results showed that CYP1A2 and CYP3A4 might be the major enzymes involved in the metabolism of cnidilin in human liver microsomes. The relationship between cnidilin and the CYP450 enzymes could provide us a theoretical basis of the pharmacological mechanism.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microssomos Hepáticos
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Medicamentos de Ervas Chinesas
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Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas em Tandem
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Compostos Heterocíclicos com 3 Anéis
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article