A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection.
BMC Genomics
; 18(1): 324, 2017 04 24.
Article
em En
| MEDLINE
| ID: mdl-28438140
ABSTRACT
BACKGROUND:
Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material.METHODS:
To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents.RESULTS:
Our approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30).CONCLUSIONS:
This method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems. TRIAL REGISTRATION (PROSPECTIVELY REGISTERED) Current Controlled Trials ISRCTN88101063 . Date of registration 14/06/2012.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus Reordenados
/
Análise de Sequência de RNA
/
Rotavirus
/
Genômica
/
Fezes
/
Genótipo
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article