Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3L1 adipocytes.
Mol Med Rep
; 15(6): 3912-3918, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28440456
ABSTRACT
Tetrahydropalmatine (THP), one of the active components of Rhizoma corydalis, has been reported to exert several pharmacological effects, including antiinflammatory, antitumor and analgesic activities. However, its effect on obesity and the underlying molecular mechanisms that may be involved have not yet been elucidated. In the present study, the inhibitory effects of THP on the adipogenesis in 3T3L1 adipocytes was examined using hstology, western blotting and RTqPCR. THP was identified to significantly suppress lipid accumulation in 3T3L1 cells and it inhibited preadipocyte differentiation in a concentrationdependent manner, as evidenced by the reduced formation of lipid droplets and decreased triglyceride levels and glycerol3phosphate dehydrogenase activity. THP downregulated the adipogenesisassociated protein and gene expressions of sterol regulatory elementbinding protein 1, fatty acid synthase, stearoylCoA desaturase 1, peroxisome proliferator activated receptor γ and CCAAT/enhancer binding proteinα in a concentrationdependent manner. In addition, it reduced adipocyte fatty acid binding protein and glycerol3phosphate acyltransferase gene expression in a concentrationdependent manner. Conversely, THP increased the mRNA expression of carnitine palmitoyltransferase 1 in a concentrationdependent manner. Furthermore, THP increased AMPactivated protein kinase (AMPK) and acetylCoA carboxylase phosphorylation in a concentrationdependent manner. These results suggested that antiadipogenic activity of TPH may be mediated via the AMPK pathway in 3T3L1 cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Alcaloides de Berberina
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Adipócitos
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Sistema de Sinalização das MAP Quinases
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Metabolismo dos Lipídeos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article