[Effect of Autophagy on Homoharringtonine-treated K562 Cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 25(2): 412-417, 2017 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-28446285
ABSTRACT
OBJECTIVE:
To study the effect of homoharringtonine (HHT) alone or combined with 3-methyladenine (3-MA) , an autophagy inhibitor, on the apoptosis and autophagy of K562 cells.METHODS:
K562 cells were treated with HHT(10 ng/ml) or HHT(10 ng/ml) combined with 3-MA (1.5 mmol/L) for 1 to 8 days. The apoptosis of treated cells was tested by means of flow cytometry(FCM), and the autophagy levels were tested with RT-PCR, Western blot and electron microscopy.RESULTS:
In the early stage of HHT-treated group, the apoptosis rate increased and decreased later. Beclin1 mRNA expression level and the LC3II/I ratio were declined firstly and increased later in HHT group. While combining with autophagy inhibitor 3-MA, both the Beclin1 mRNA expression level and the LC3II/I ratio were declined continually during the treated period. The activated caspase-3 protein expression level was also raised sustainability during both HHT and 3-MA cultured period.CONCLUSIONS:
HHT can induce apoptosis of K562 cells, but the sustaining effect of HHT can induc autophagy of K562 cells, the combination of HHT with 3-MA may enhance the cytotoxicitic effect of HHT on K562 cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Harringtoninas
/
Antineoplásicos Fitogênicos
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article