Switchable Cas9.
Curr Opin Biotechnol
; 48: 119-126, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-28456061
ABSTRACT
Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post-translational stage via external trigger signals. Control is either achieved by modifying the Cas9 protein itself or its programmable RNA molecules. To date, switchable Cas9 variants responding to small ligands, light or temperature have been engineered. With these variants in hand, the regulation and modification of genomes can be accomplished in graded and ever more precise manner.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Genoma Humano
/
Regulação da Expressão Gênica
/
Proteínas Associadas a CRISPR
/
Sistemas CRISPR-Cas
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article