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Metabolic flux network analysis of hydrogen production from crude glycerol by Clostridium pasteurianum.
Sarma, Shyamali; Anand, Avinash; Dubey, Vikash Kumar; Moholkar, Vijayanand S.
Afiliação
  • Sarma S; Centre for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
  • Anand A; Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
  • Dubey VK; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
  • Moholkar VS; Centre for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India. Electronic address: vmoholkar@iitg.ernet.in.
Bioresour Technol ; 242: 169-177, 2017 Oct.
Article em En | MEDLINE | ID: mdl-28456454
The present study has attempted to get insight into ultrasound induced enhancement in biohydrogen production from glycerol fermentation using metabolic flux analysis (MFA). A pseudo steady state metabolic flux network model was constructed and analyzed using experimentally measured glycerol uptake rate and fluxes of four metabolites, viz. acetate, butyrate, succinate and 1,3-PDO. Glycerol consumption increased by ∼50% under sonication. Biohydrogen yield showed marked rise of ∼40% with application of ultrasound. Butyrate and 1,3-PDO were the major products of glycerol metabolism. Sonication had major influence on carbon fluxes at the acetyl-CoA node. MFA results revealed enhanced flux towards butyrate under sonication, which was manifested in higher butyrate to acetate (B/A) ratio in products and greater H2 generation. Biohydrogen production was also a microbial growth associated process. Finally, two theoretical alternatives for further enhancement of biohydrogen production were assessed with MFA, viz. enhancement of glycerol uptake and blocking of butyrate pathway.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clostridium / Glicerol Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clostridium / Glicerol Idioma: En Ano de publicação: 2017 Tipo de documento: Article